Organoleptic characteristics and compositional profile of meat of growing Japanese quail fed different levels of poultry byproducts compost.

The poultry industry generates a lot of waste, including dead birds, manure, and poultry litter. Poultry waste should never be disposed of improperly because it can seriously harm the environment. The waste can be recycled as a feedstock for use in poultry feed by composting the litter and deceased birds. The compositional profile and organoleptic properties of the meat of growing Japanese quail were examined over the course of a 4-week trial to ascertain the effect of adding compost to the diet. In a completely randomized design (CRD), 1200 newly hatched quail chicks (Coturnix coturnix japonica) were divided into five treatment groups (diets with 0, 2.5, 5, 7.5, and 10% compost), each consisting of 40 birds with six replicates. The addition of compost to the diet had no noticeable effects on the organoleptic qualities of appearance, color, aroma, taste, texture, juiciness, tenderness, and acceptability (P>0.05). The compositional profile characteristics for chicks given compost at any level compared to chicks fed the control diet showed no differences (P>0.05). These findings suggest that the sensory characteristics and compositional profile of growing meat quails can be maintained when fed diets including up to 10% compost.

Assessing effect of feeding poultry byproducts compost on organoleptic characteristics and compositional profile of meat of broiler chickens.

Large amounts of waste, including dead birds, manure, and poultry litter, are produced by the poultry industry. Poultry waste should be disposed of properly to avoid major pollution and health risks. Composting litter and dead birds could be an option to recycle the waste and use in poultry feed. A study was conducted to investigate the effects of feeding composted poultry waste on the organoleptic qualities and compositional profile of the meat of broiler chickens. A total of 300 day-old broiler chicks (500-Cobb) were randomly allocated to five treatment groups replicated six times with 10 birds each, under a completely randomized design (CRD). Five iso-caloric and iso-nitrogenous diets including composted poultry byproducts at concentrations of 0, 2.5, 5, 7.5, and 10% were fed ad libitum to the birds from day 0 to day 35. The sensory grading and meat composition profile of 500 Cobb broiler chickens were tested at 35 days of age. The findings showed that there were no variations in the sensory profiles of the meat from birds given various diets (P>0.05). Although the results were somewhat lower for the chicks fed compost-containing diets than for the control group, this difference was deemed to be insignificant (P>0.05). Similarly, there were no variations in the compositional profile values of the meat between meat from birds fed various diets (P>0.05). These findings imply that broiler chickens may be raised on diets containing up to 10% poultry byproduct compost without any negative impacts on the meat's sensory quality or composition. Additionally, using compost into broiler diets may help to lower the cost of feed.

Quantitative analysis of human hairs and nails.

Hair and nails are human biomarkers capable of providing a continuous assessment of the concentrations of elements inside the human body to indicate the nutritional status, metabolic changes, and the pathogenesis of various human diseases. Laser-induced breakdown spectroscopy (LIBS) and X-ray fluorescence (XRF) spectrometry are robust and multi-element analytical techniques able to analyze biological samples of various kinds for disease diagnosis. The primary objective of this review article is to focus on the major developments and advances in LIBS and XRF for the elemental analysis of hair and nails over the last 10-year period. The developments in the qualitative and quantitative analyses of human hair and nail samples are discussed in detail, with special emphasis on the key aspects of elemental imaging and distribution of essential and non-essential elements within the hair and nail tissue samples. Microchemical imaging applications by LIBS and XRF (including micro-XRF and scanning electron microscopy, SEM) are also presented for healthy as well as diseased tissue hair and nail samples in the context of disease diagnosis. In addition, main challenges, prospects, and complementarities of LIBS and XRF toward analyzing human hair and nails for disease diagnosis are also thoroughly discussed here.

Photocatalytic deactivation of sulphate reducing bacteria using visible light active CuO/TiO2 nanocomposite photocatalysts synthesized by ultrasonic processing.

Sulphate-reducing bacteria wreaks havoc to oil pipelines, as it is an active agent for scale formation in the oil production tubing, and plugging of reservoir rock around the oil wells, and this leads to the degradation of oil quality. In this work, we synthesized copper oxide/titanium dioxide nanocomposite photocatalysts with three different mass contents of copper oxide (10%, 20% and 30%) and used them as an effective photo-catalyst in the process of photo-catalytic deactivation of sulphate-reducing bacteria. The anchoring of copper oxide on titanium dioxide brought about the following positive attributes in copper oxide/titanium dioxide nanocomposite pertained to the photo-catalyst: (i) the material transformed to visible light active with the potential to harness the more efficient visible spectral region of the solar radiation, (ii) increased surface area on the photo-catalyst enhanced the number of active reaction sites in the material, and (iii) efficiently retarded the undesired photo-generated electron hole recombination to promote the photo-catalytic activity. Although, the photo-catalyst effective under both UV and visible light, the deactivation was found to be higher in visible radiation, particularly the nanocomposite with 20%- copper oxide on titanium dioxide showed the highest photocatalytic degradation with of Sulphate-reducing bacteria with a decay constant as high as 1.38 min -1 and the total depletion time as low as 8 min. It was confirmed that the bacterial deactivation was neither due to the bactericidal effect of the nanocomposite nor due to the light mediated deactivation.

Role of vanadium ions substitution on spinel MnCo2O4 towards enhanced electrocatalytic activity for hydrogen generation.

Improving efficient electrocatalysts (ECs) for hydrogen generation through water splitting is of significant interest in tackling the upcoming energy crisis. Sustainable hydrogen generation is the primary prerequisite to realizing the future hydrogen economy. This work examines the electrocatalytic activity of hydrothermally prepared vanadium doped MnCo spinel oxide microspheres (MC), MnVxCo2-xO4 (Vx-MnCo MC, where x ≤ 0.4) in the HER (hydrogen evolution reaction) process. Magnetization measurements demonstrated a paramagnetic (at high temperatures) to a ferrimagnetic (at low temperatures) transition below the Curie temperature (Tc) in all the samples. The magnetization is found to intensify with the rising vanadium content of MCs. The optimized catalyst Vx-MnCo MCs (x = 0.3) outperformed other prepared ECs with a Tafel slope of 84 mV/dec, a low onset potential of 78.9 mV, and a low overpotential of 85.9 mV at a current density of 10 mA/cm2, respectively. The significantly improved HER performance of hydrothermally synthesized Vx-MnCo MCs (x = 0.3) is principally attributable to many exposed active sites, accelerated electron transport at the EC/electrolyte interface, and remarkable electron spectroscopy for chemical analysis (ECSA) value was found ~ 11.4 cm2. Moreover, the Vx-MnCo MCs (x = 0.3) electrode exhibited outstanding electrocatalytic stability after exposure to 1000 cyclic voltametric cycles and 36 h of chronoamperometric testing. Our results suggest a feasible route for developing earth-abundant transition metal oxide-based EC as a superior electrode for future water electrolysis applications.

Effects of selenium supplementation on the growth performance, slaughter characteristics, and blood biochemistry of naked neck chicken.

This study examined how selenium-supplemented diets affected the performance of naked neck chickens. The birds were fed both organic and inorganic selenium at 0.30 ppm, while the control diet did not include any additional selenium. A total of 225 one-day-old naked neck chicks were randomly divided into 3 experimental groups, each of which was replicated 5 times (replicates) and contained 15 birds. This was done using a completely randomized design. The data was collected after growth, meat quality, and blood profile parameters were assessed. The findings showed that the birds fed inorganic selenium in the diet displayed increased (P < 0.05) feed intake followed by those administered organic selenium and the control diet. On the other hand, birds fed organic selenium in the diet showed enhanced body weight gain and better feed conversion ratio (P < 0.05). Similarly, organic selenium supplementation increased (P < 0.05) breast and thigh weight compared to inorganic selenium, but no other metrics, such as dressing percentage, drumstick weight, liver weight, gizzard weight, heart weight, or wing weight, significantly differed between treatments (P > 0.05). When compared to birds fed inorganic Se and control diet, the birds fed organic Se had greater (P < 0.05) blood levels of total protein and globulin. Additionally, it was discovered that organic Se-fed birds had greater (P < 0.05) blood Se concentrations than control and inorganic Se-fed birds. However, no differences between treatments were found in albumin, glucose, cholesterol, triglycerides, or uric acid (P > 0.05). In conclusion, adding Se-enriched yeast, as an organic selenium source, to diets may enhance the poor growth and slaughter characteristics of naked neck chicks without negatively affecting blood chemistry.

Customization of surface wettability of nano-SiO2 by coating Trimethoxy(vinyl)silane modifier for oil-water separation: Fabrication of metal-based functional superwetting nanomaterial, characterizations and performance evaluation.

The wettability of nano-SiO2 surface was transformed from the inherent hydrophilicity to functional superhyderophobicity by coating Trimethoxy (vinyl)silane modifier, and the resultant surface showed contrasting wettability for water and oil (Superhydrophobic and Superoleophilic), which is a desired characteristic for the membranes used in oil-water separation. Initially Trimethoxy (vinyl)silane coated SiO2 nanoparticles (TMVS@SiO2) were synthesized by hydrolysis and poly-condensation reactions, and this nano dispersion was spray coated on the annealed stainless-steel mesh surface, whose resulting hierarchical surface texture brought about the desired wettability, with the water-surface-air (θWA) and oil-surface-air (θOA) interfacial contact angles of 150° and 0° respectively. In addition to the wettability studies (contact angles), FTIR, morphological, and elemental characterizations of the TMVS@SiO2 coated surfaces were carried out to understand the alterations that have taken place on the TMVS@SiO2 surface that in turn rendered superhydrophobicity and superoleophilicity to the surface. The FTIR absorption peaks indicate that after modifying SiO2 with TMVS, the -OH groups on SiO2 surface are clearly replaced by -CH3. The morphological studies indicated that modification of SiO2 leads to better cross-linking between coating composition and nanoparticles and EDS spectra and elemental mapping of the modified surface showed the presence of Si, O and C elements. Finally, this surface was tested for its efficiency and stability as a membrane in the process of separating oil and water from the oily water using gravity driven method. The oil-water separation efficiency was estimated to be 99% for this membrane and also it was found to be quite stable as the surface effectively retained this oil-water separation efficiency even after 10 cycles of separation process.

Assessing growth performance, morphometric traits, meat chemical composition and cholesterol content in four phenotypes of naked neck chicken.

The present study was conducted to assess the growth performance, morphometric traits, muscle chemical composition and cholesterol content in four phenotypes of naked neck chicken (black, white-black, light brown and dark brown). A total of 320-day-old chicks, 80 from each phenotype, were randomly stratified into 20 replicates (16/replicate), according to a completely randomized design. The results showed higher final body weight, weight gain, and better FCR in both light brown and dark brown phenotypes whereas time of gains was found to be greater in dark brown phenotype. Keel length and shank circumference were greater in dark brown whereas wing spread was found to be higher in light brown phenotype. Drumstick circumference and body length did not show any significant differences (P > 0.05) across the phenotypes. Dry matter was found to be higher in white black, crude protein in black, white black and dark brown, moisture in light brown, and cholesterol content in black whereas ether extract and ash content were found to be greater in black and white black phenotypes. In conclusion, both light brown and dark brown phenotypes showed superior growth performance and morphometric traits. Similarly, from a health point of view, the dark brown and light brown phenotypes seem superior because their cholesterol content was low. Thus, it is strongly recommended that there should be a conscious effort to improve economically important traits of the light and dark brown birds to be used as dual-purpose slow growing chicken, especially in developing countries.

Rapid Determination and Quantification of Nutritional and Poisonous Metals in Vastly Consumed Ayurvedic Herbal Medicine (Rejuvenator Shilajit) by Humans Using Three Advanced Analytical Techniques.

Shilajit is used commonly as Ayurvedic medicine worldwide which is Rasayana herbo-mineral substance and consumed to restore the energetic balance and to prevent diseases like cognitive disorders and Alzheimer. Locally, Shilajit is applied for patients diagnosed with bone fractures. For safety of the patients, the elemental analysis of Shilajit is imperative to evaluate its nutritional quality as well as contamination from heavy metals. The elemental composition of Shilajit was conducted using three advanced analytical techniques (LIBS, ICP, and EDX). For the comparative studies, the two Shilajit kinds mostly sold globally produced in India and Pakistan were collected. Our main focus is to highlight nutritional eminence and contamination of heavy metals to hinge on Shilajit therapeutic potential. In this work, laser-induced breakdown spectroscopy (LIBS) was applied for qualitative and quantitative analysis of the Shilajit. Our LIBS analysis revealed that Shilajit samples composed of several elements like Ca, S, K, Mg, Al, Na, Sr, Fe, P, Si, Mn, Ba, Zn, Ni, B, Cr, Co, Pb, Cu, As, Hg, Se, and Ti. Indian and Pakistani Shilajits were highly enriched with Ca, S, and K nutrients and contained Al, Sr, Mn, Ba, Zn, Ni, B, Cr, Pb, As, and Hg toxins in amounts that exceeded the standard permissible limit. Even though the content of most elements was comparable among both Shilajits, nutrients, and toxins, in general, were accentuated more in Indian Shilajit with the sole detection of Hg and Ti. The elemental quantification was done using self-developed calibration-free laser-induced breakdown spectroscopy (CF-LIBS) method, and LIBS results are in well agreement with the concentrations determined by standard ICP-OES/MS method. To verify our results by LIBS and ICP-OES/MS techniques, EDX spectroscopy was also conducted which confirmed the presence above mentioned elements. This work is highly significant for creating awareness among people suffering due to overdose of this product and save many human lives.

Development of laser induced breakdown spectroscopy technique to study irrigation water quality impact on nutrients and toxic elements distribution in cultivated soil.

This study is focused mainly on impact of irrigation water quality in cultivated soil on distribution of essentials nutrients (Al, Mg, Ca, Fe, S, Si, Na, P, and K) and relatively toxic metals (As, Ba, Cr, Cu, Ti, Sn, Mn, Ni, and Zn) using an elegant Laser induced breakdown spectroscopy (LIBS) technique. A pulsed Nd:YAG laser operating at 1064 nm in conjunction with suitable detector was applied to record soil emission spectra. The abundance of these elements were evaluated via standard calibration curve Laser Induced Breakdown Spectroscopy (CC-LIBS) and calibration free Laser Induced Breakdown Spectroscopy (CF-LIBS) approaches. Quantitative analyses were accomplished under conjecture of local thermodynamic equilibrium (L.T.E) and optically thin plasma. The average electron temperatures were estimated by Boltzmann plot method for cultivated soil samples in 7800 to 9300 K range. The electron number density was ~ 1.11 × 1017 cm - 3 to 1.60 × 1017 cm - 3. Prior to application on soil samples, the experimental setup was optimized at the following parameters: pulsed energy = 60 mJpulse-1, sample to lens distance of 9.0 cm, and the gate delay of 3.5 µs. It is noteworthy that nutritional elements content of cultivated soils were found strongly dependent upon the irrigation water quality. The cultivated soil from industrial area was found rich of toxins while the cultivated land using tube well water contains toxins in least amount. Our LIBS findings were also validated by comparing its results with contents measured using a standard inductively coupled plasma optical emission spectroscopy (ICP-OES) method and both were found in excellent agreement. The present study could be highly beneficial for agricultural applications and for farmers to produce safe food products and higher crops yield.

Linking fish and crustacean taxonomic composition with seasonal contrasts in the soft-bottom intertidal zone / Ligação da composição taxonômica de peixes e crustáceos com contrastes sazonais na zona intertidal de fundo macio

Abstract The primary objective of this study was to investigate the seasonal fish and crustacean variations concerning taxonomic composition, species richness, and diversity in sandy beach habitat. For this purpose, we investigated the Sonmiani Hor lagoon area during four distinct seasons, i.e., northeast (NE) monsoon, pre-monsoon, south-west (SW) monsoon, and post-monsoon for one year. During each haul, the net was pulled about 100m along the beach in 0.5m depth. The results showed a strong linear correlation between the diversity index and equitability in fishes (r = 0.978). The diversity index was strong negatively correlated with the abundance and biomass (r = -0.978, -0.972, respectively). The physical attributes like sea surface water temperature and salinity showed a strong negative effect on species assemblages (r = -0.981 and -0.943, respectively). The mean air and water temperature illustrated approximately 3°C difference during NE and pre-monsoon seasons. However, salinity, pH, and electrical conductivity did not show any significant seasonal variabilities. Under the ecological indices, the fish species displayed higher diversity (H' = 3.19) during SW monsoon, whereas the lowest diversity was observed during pre-monsoon (H' = 1.58). The equitability and species richness, however, remained more noticeable during SW monsoon (J' = 0.81). The total number of individuals of fish and crustaceans reached 4799 with 3813 fish individuals and 986 individuals of crustaceans. A total of 27 families of fish while five crustacean families comprising of 30 genera and 38 fish species while ten genera and 17 species of crustaceans were recorded. Liza subviridis displayed the highest abundance among the sampled fish species. In conclusion, fish species constituted a significant part of the coastal fauna in the study area. The seasonal variations displayed distinct variations in fish species composition and diversity.

Resumo O objetivo principal deste estudo foi investigar as variações sazonais de peixes e crustáceos em relação à composição taxonômica, riqueza de espécies e diversidade no habitat de praias arenosas. Para esse fim, investigamos a área da lagoa Sonmiani Hor durante quatro estações distintas, ou seja, monção nordeste (NE), pré-monção, monção sudoeste (SW) e pós-monção, por um ano. Durante cada transporte, a rede foi puxada cerca de 100 m ao longo da praia, a 0,5 m de profundidade. Os resultados mostraram uma forte correlação linear entre o índice de diversidade e a equitabilidade de peixes (r = 0,978). O índice de diversidade apresentou forte correlação negativa com a abundância e a biomassa (r = -0,978 e -0,972, respectivamente). Os atributos físicos, como temperatura da água da superfície do mar e salinidade, apresentaram forte efeito negativo sobre o conjunto de espécies (r = -0,981 e -0,943, respectivamente). A temperatura média do ar e da água teve uma diferença de aproximadamente 3 °C durante a monção NE e a pré-monção. No entanto, salinidade, pH e condutividade elétrica não apresentaram variabilidade sazonal significativa. A respeito dos índices ecológicos, as espécies de peixes apresentaram maior diversidade (H' = 3,19) durante a monção SW, enquanto a menor diversidade observada foi na pré-monção (H' = 1,58). A equitabilidade e a riqueza de espécies, no entanto, permaneceram mais perceptíveis durante a monção SW (J' = 0,81). O número total de peixes e crustáceos foi de 4.799, com 3.813 peixes e 986 crustáceos. Foram registradas 27 famílias de peixes e 5 famílias de crustáceos, com 30 gêneros e 38 espécies de peixes, além de 10 gêneros e 17 espécies de crustáceos. Liza subviridis apresentou a maior abundância entre as espécies de peixes amostradas. Em conclusão, as espécies de peixes constituíram uma parte significativa da fauna costeira na área de estudo. As variações sazonais apresentaram variações distintas na composição e diversidade das espécies de peixes.

Nutritional and toxic elemental analysis of dry fruits using laser induced breakdown spectroscopy (LIBS) and inductively coupled plasma atomic emission spectrometry (ICP-AES).

Quantitative investigation of essential and trace heavy elements present in health-beneficial dry fruits (Pistachio, Almonds, Black walnut, White walnut, and Cashew) was investigated using Laser Induced Breakdown Spectroscopy. For an accurate elemental exposure using LIBS technique, the local thermo-dynamical equilibrium of the laser induced plasma was established and verified using McWhirter criterion based on the electron number density in the plasma. Earlier to engage, our LIBS detector was optimized. For quantification of elements, standard calibration curves (CC)-LIBS method was applied. Using our LIBS system, the nutritional elements such as Al, Mg, Ca, Fe, K, Zn, and Na and toxins like Pb, Cr, and Cu were detected in dry fruits. The elemental quantification of dry fruit contents were validated using standard (ICP-AES) method and the relative accuracy of our experimental setup in comparison to ICP approach was in the ranging from 0.1 to 0.3 at 2.5-% error confidence.

Linking fish and crustacean taxonomic composition with seasonal contrasts in the soft-bottom intertidal zone.

The primary objective of this study was to investigate the seasonal fish and crustacean variations concerning taxonomic composition, species richness, and diversity in sandy beach habitat. For this purpose, we investigated the Sonmiani Hor lagoon area during four distinct seasons, i.e., northeast (NE) monsoon, pre-monsoon, south-west (SW) monsoon, and post-monsoon for one year. During each haul, the net was pulled about 100m along the beach in 0.5m depth. The results showed a strong linear correlation between the diversity index and equitability in fishes (r = 0.978). The diversity index was strong negatively correlated with the abundance and biomass (r = -0.978, -0.972, respectively). The physical attributes like sea surface water temperature and salinity showed a strong negative effect on species assemblages (r = -0.981 and -0.943, respectively). The mean air and water temperature illustrated approximately 3°C difference during NE and pre-monsoon seasons. However, salinity, pH, and electrical conductivity did not show any significant seasonal variabilities. Under the ecological indices, the fish species displayed higher diversity (H' = 3.19) during SW monsoon, whereas the lowest diversity was observed during pre-monsoon (H' = 1.58). The equitability and species richness, however, remained more noticeable during SW monsoon (J' = 0.81). The total number of individuals of fish and crustaceans reached 4799 with 3813 fish individuals and 986 individuals of crustaceans. A total of 27 families of fish while five crustacean families comprising of 30 genera and 38 fish species while ten genera and 17 species of crustaceans were recorded. Liza subviridis displayed the highest abundance among the sampled fish species. In conclusion, fish species constituted a significant part of the coastal fauna in the study area. The seasonal variations displayed distinct variations in fish species composition and diversity.

Evaluation of bioactivities of zinc oxide, cadmium sulfide and cadmium sulfide loaded zinc oxide nanostructured materials prepared by nanosecond pulsed laser.

The present study reports the preparation of cadmium sulfide (CdS) loaded zinc oxide (ZnO) nanostructured semiconductor material and its anti-bioactivity studies against cancerous and fungus cells. For composite preparation, two different mass ratios of CdS (10 and 20%) were loaded on ZnO (10%CdS/ZnO, 20%CdS/ZnO) using a 532 nm pulsed laser ablation in water media. The structural and morphological analyses confirmed the successful loading of nanoscaled CdS on the surface of ZnO particles, ZnO particles were largely spherical with average size ~50 nm, while CdS about 12 nm in size. The elemental and electron diffraction analyses reveal that the prepared composite, CdS/ZnO contained both CdS and ZnO, thus reaffirming the production of CdS loaded ZnO. The microscopic examination and MTT assay showed the significant impact of ZnO, CdS, and CdS loaded ZnO on human colorectal carcinoma cells (HCT-116 cells). Our results show that the prepared ZnO had better anticancer activities than individual CdS, and CdS loaded ZnO against cancerous cells. For antifungal efficacy, as-prepared nanomaterials were investigated against Candida albicans by examining minimum inhibitory/fungicidal concentration (MIC/MFC) and morphogenesis. The lowest MIC (0.5 mg/mL), and MFC values (1 mg/mL) were found for 10 and 20%CdS/ZnO. Furthermore, the morphological analyses reveal the severe damage of the cell membrane upon exposure of Candida strains to nanomaterials. The present study suggests that ZnO, CdS, and CdS loaded ZnO nanostructured materials possess potential anti-cancer and anti-fungal activities.

Single step production of high-purity copper oxide-titanium dioxide nanocomposites and their effective antibacterial and anti-biofilm activity against drug-resistant bacteria.

In the present research work, copper oxide-titanium dioxide nanocomposites were synthesized for the first time using advanced pulsed laser ablation in liquid (PLAL) technique for disinfection of drug-resistant pathogenic waterborne biofilm-producing bacterial strains. For this, a series of copper oxide-titanium dioxide nanocomposites were synthesized by varying the composition of copper oxide (5%, 10%, and 20%) with titanium dioxide. The pure titanium dioxide and copper oxide-titanium dioxide nanocomposites were characterized by advanced instrumental techniques. XRD, TEM, FE-SEM, EDX, elemental mapping and XPS analysis results consistently revealed the successful formation of copper oxide-titanium dioxide nanocomposites using PLAL technique. The antibacterial and antibiofilm activities of pure titanium dioxide and copper oxide-titanium dioxide nanocomposites were investigated against biofilm-producing strains of Methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa by various methods. Our results revealed that the PLAL synthesized copper oxide-titanium dioxide nanocomposites showed enhanced anti-biofilm and antibacterial activity compared to pure titanium dioxide in a dose-dependent manner against targeted pathogens. Furthermore, the effects of pure titanium dioxide and copper oxide-titanium dioxide nanocomposites on bacterial morphology, biofilm formation, aggregation and their colonization by targeted pathogens were also examined using scanning electron microscopy. Microscopic images clearly showed that the cell envelope of almost all the cells were rumples, rough, had irregularities and abnormal appearance with the major damage being characterized by the formation of "pits". Many depressions and indentations were also seen in their cell envelope and the original shape of Pseudomonas aeruginosa cells changed from normal rod to swollen, large and elongated which indicates the loss of membrane integrity and damage of cell wall and membrane. The findings suggested that PLAL synthesized copper oxide-titanium dioxide nanocomposites have good potential for removal of biofilm or killing of pathogenic bacteria in water distribution network and for wastewater treatment, hospital and environmental applications. In addition, cytotoxic activity of pure TiO2 and PLAL synthesized copper oxide-titanium dioxide nanocomposites against normal and healthy cells (HEK-293) and cancerous cells (HCT-116) were also evaluated by MTT assay. The MTT assay results showed no cytotoxic effects on HEK-293 cells, which suggest TiO2 and PLAL synthesized copper oxide-titanium dioxide nanocomposites are non-toxic to the normal cells.

Spectral analysis of Miracle Moringa tree leaves using X-ray photoelectron, laser induced breakdown and inductively coupled plasma -optical emission spectroscopic techniques.

The antioxidant Moringa oleifera (a medicinal plant) leaves (MOLs) containing diverse nutrients are highly beneficial for the human health. The MOLs upon consumption can lower the blood sugar, cure the heart diseases, and reduce the inflammation. In this perception, the "primary nutrients contents" in the dry MOLs (pellet samples) were evaluated for the first time using the XPS, LIBS and ICP-OES techniques. The XPS analysis of the MOLs showed the presence of vital elements like calcium (Ca), magnesium (Mg), manganese (Mn), copper (Cu), phosphorous (P), sulfur (S) and zinc (Zn). The LIBS analyses of the MOLs revealed the atomic and ionic spectral lines corresponding to the essential nutrients such as the Ca, Na, K, Fe, Mg, Mn, Cu, P, S and Zn. The calibration free LIBS algorithm (CF-LIBSA) was developed to quantify the content of each element in the dry MOLs. In addition, the LIBS results were validated by the analysis using ICP-OES standard analytical technique. The elemental contents in the MOLs obtained from the CF-LIBS analyses were counter verified by the ICP-OES results. Present results are highly valuable for the development of a traditional herbal medicine using the miracle MOLs.

Spectral diagnosis of health hazardous toxins in face foundation powders using laser induced breakdown spectroscopy and inductively coupled plasma-optical emission spectroscopy (ICP-OES).

In this work, we applied laser-induced breakdown spectroscopy (LIBS) to estimate the amount of toxic heavy metals (Pb, Cr, Ni) in five different brands of face foundation powders available in the local market using standard calibration curve (CC)-LIBS approach. These samples contain toxic elements like lead, chromium, nickel which are highly carcinogens to cause eczematous and atopic dermatitis. The strongest atomic transition lines of lead (405.7 line), chromium (425.4 nm line), and nickel (336.9 nm line) were used as spectral markers to simultaneously detect these three heavy metals in face powder samples. The LIBS experimental parameters (delay time, laser energy, and focusing lens to target spacing) were optimized to enhance the signal to noise ratio in the resulting LIBS spectra. The consequence of self-absorption on the marker peaks used in the LIBS analysis was investigated and found to be insignificant. We calibrated our LIBS system for these three heavy metals, whose levels were found above the permissible limits. The face foundation powder used every day augments up to considerable exposure of heavy metals in the human body which could cause many skin disorders. Our LIBS quantitative results were also cross-validated by analysis using a standard analytical technique known as inductively coupled plasma-optical emission spectroscopy (ICP-OES). This study is highly significant due to the excessive use of cosmetic products especially face powder that could affect the health of millions of people around the globe.

Determination of heavy metals in cancerous and healthy colon tissues using laser induced breakdown spectroscopy and its cross-validation with ICP-AES method.

Certain types of cancer deaths are widespread due to the diagnostic complexities at the early stages. Recent studies revealed that the bio-accumulated heavy metals (act as pro-carcinogens) in the human colon cause strong interactions with the nuclear proteins, leading to the DNA damages and repair processes inhibition, resulting in the cancers. Most of the conventional detection techniques suffer from the insensitiveness, cost-intensiveness, imprecision, slowness, cumbersomeness of sample preparation, and conflicting outcomes. To surmount these shortcomings an accurate, reliable, and rapid detection technique is essential for the early diagnostic and prevention of heavy metals accumulation induced colon cancers. This will enable not only to classify the malignant and non-malignant cells but can differentiate the proteins from abnormal cellular morphology. Using the indigenously developed fast and accurate (calibration-free) laser-induced breakdown spectroscopy (LIBS), we analyzed several cancerous and normal colon tissues collected from the colon cancer infected patients aged 40-60 years. The results showed the presence of carcinogenic heavy metals including lead (Pb), chromium (Cr), and mercury (Hg) in the malignant colon tissues, while the healthy tissues were devoid of these elements. The concentrations of Pb, Cr and Hg in the cancerous samples were discerned to be 3.1, 13.4 and 7.1 µg/L, respectively. Furthermore, the performance accuracy of the LIBS technique was validated by comparing the results obtained from the standard inductively coupled plasma atomic emission spectroscopy (ICP-OES). It was established that the LIBS analysis is useful for the speedy, precise and early detection of the heavy metals amassed malignant colon tissues, thereby saving millions of lives by promoting the chances for cancer cure.

Synthesis, characterization and evaluation of visible light active cadmium sulfide-graphitic carbon nitride nanocomposite: A prospective solar light harvesting photo-catalyst for the deactivation of waterborne pathogen.

Cadmium sulfide-graphitic carbon nitride nanocomposite was synthesized by pulsed laser ablation in liquid, and it was found from the results of optical and morphological characterizations that the proper anchoring of nanostructured cadmium sulfide on the nano-sheets of graphitic carbon nitride took place, which brought about the positive attributes such as enhanced visible light absorption and reduced photo-generated charge recombination, the key features required for an efficient photo-catalyst by solar light harvesting. The pulsed laser ablation in liquid method adopted for the synthesis of cadmium sulfide-graphitic carbon nitride has the following advantages: the shape and size of the synthesized particles can be controlled by altering the experimental parameters such as laser wavelength, pulse laser duration, the pH of the solution, the surfactants and the temperature of the solution, pulsed laser ablation in liquid method neither requires cumbersome equipment nor does it require intermediate chemicals and catalysts nor does it necessitate the post synthesis purification. The enhancement of photo-catalytic activity of cadmium sulfide-graphitic carbon nitride nanocomposite was tested for the photo-catalytic deactivation of Escherichia coli bacteria in water under visible light radiation. As anticipated, a significant improvement of photo-catalytic deactivation was observed, which is attributed to the enhanced and extended light absorption in the visible spectral region, and the formation of herterojunction between the semiconductors, which is instrumental in inhibiting the undesired recombination of photo-generated charge carriers. Quantitatively, the presence of cadmium sulfide on the graphitic carbon nitride surface contributed to a remarkable 129% increase of photo-catalytic degradation constant compared to pure graphitic carbon nitride, which resulted in the decrease of total depletion time of Escherichia coli from 156 min to 67 min with the cadmium sulfide-graphitic carbon nitride nanocomposite synthesized by pulsed laser ablation in liquid method. Our results on the efficient photo-catalytic deactivation of Escherichia coli under visible light assures that cadmium sulfide-graphitic carbon nitride nanocomposite can very well be used for photo-catalytic water purification by harvesting the abundant solar light.

LIBS for the detection of lead in ready to use henna paste and nutrients in fresh henna leaves and cultivated soils.

Commercially available ready to use henna paste, procured from the local market in Pakistan were tested to find the level of hazardous element, lead present in the samples, using Laser Induced Breakdown Spectroscopy (LIBS). The source of lead in the ready to use henna is from the artificial chemical additives in henna paste, added to enhance the color and also to speed up the dying of the henna tattoo in hand, feet, and body. Henna tattooing is an inevitable practice in the social events in the cultures of the Indian subcontinent and the Arabian Peninsula. The frequent reckless use of henna, particularly lavishly administering it to the children is quite dangerous, although the level of lead found in the commercially available ready to use henna samples are within the permissible safe level. For the sensitive detection and quantification of lead, various experimental and plasma parameters in the LIBS detection system were optimized and the system was calibrated. The quantitative analysis of lead, carried out in five different samples of ready to use henna, using LIBS revealed that the concentration of lead in these samples is between 5.5 ±â€¯0.2 mg Kg-1 and 16.0 ±â€¯0.2 mg Kg-1. Moreover, LIBS elemental analysis of natural henna leaves, cultivated in Sindh and Punjab regions of Pakistan, and also the soils taken from the locations of cultivation were carried out and a correlation in the elemental compositions between the henna leaves and the cultivated soil in the proximity were studied. The quantitative analysis of LIBS results were validated by atomic absorption spectroscopy (AAS) and inductively coupled plasma-optical emission spectroscopy (ICP-OES).